Physiol Rev AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Physiol. Rev. 81: 1659-1688, 2001;
0031-9333/01 $15.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (138)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Menon, S. T.
Right arrow Articles by Sakmar, T. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Menon, S. T.
Right arrow Articles by Sakmar, T. P.

Physiological Reviews, Vol. 81, No. 4, October 2001, pp. 1659-1688
Copyright ©2001 by the American Physiological Society

Rhodopsin: Structural Basis of Molecular Physiology

Santosh T. Menon, May Han, and Thomas P. Sakmar

Howard Hughes Medical Institute, Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York

Menon, Santosh T., May Han, and Thomas P. Sakmar. Rhodopsin: Structural Basis of Molecular Physiology. Physiol. Rev. 81: 1659-1688, 2001.The crystal structure of rod cell visual pigment rhodopsin was recently solved at 2.8-Å resolution. A critical evaluation of a decade of structure-function studies is now possible. It is also possible to begin to explain the structural basis for several unique physiological properties of the vertebrate visual system, including extremely low dark noise levels as well as high gain and color detection. The ligand-binding pocket of rhodopsin is remarkably compact, and several apparent chromophore-protein interactions were not predicted from extensive mutagenesis or spectroscopic studies. The transmembrane helices are interrupted or kinked at multiple sites. An extensive network of interhelical interactions stabilizes the ground state of the receptor. The helix movement model of receptor activation, which might apply to all G protein-coupled receptors (GPCRs) of the rhodopsin family, is supported by several structural elements that suggest how light-induced conformational changes in the ligand-binding pocket are transmitted to the cytoplasmic surface. The cytoplasmic domain of the receptor is remarkable for a carboxy-terminal helical domain extending from the seventh transmembrane segment parallel to the bilayer surface. Thus the cytoplasmic surface appears to be approximately the right size to bind to the transducin heterotrimer in a one-to-one complex. Future high-resolution structural studies of rhodopsin and other GPCRs will form a basis to elucidate the detailed molecular mechanism of GPCR-mediated signal transduction.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Mahalingam, K. Martinez-Mayorga, M. F. Brown, and R. Vogel
Two protonation switches control rhodopsin activation in membranes
PNAS, November 18, 2008; 105(46): 17795 - 17800.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Peralvarez-Marin, V. A. Lorenz-Fonfria, R. Simon-Vazquez, M. Gomariz, I. Meseguer, E. Querol, and E. Padros
Influence of Proline on the Thermostability of the Active Site and Membrane Arrangement of Transmembrane Proteins
Biophys. J., November 1, 2008; 95(9): 4384 - 4395.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
B. Isin, K. Schulten, E. Tajkhorshid, and I. Bahar
Mechanism of Signal Propagation upon Retinal Isomerization: Insights from Molecular Dynamics Simulations of Rhodopsin Restrained by Normal Modes
Biophys. J., July 15, 2008; 95(2): 789 - 803.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
L. Oliveira, C. M. Costa-Neto, C. R. Nakaie, S. Schreier, S. I. Shimuta, and A. C. M. Paiva
The Angiotensin II AT1 Receptor Structure-Activity Correlations in the Light of Rhodopsin Structure
Physiol Rev, April 1, 2007; 87(2): 565 - 592.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Abaffy, A. Malhotra, and C. W. Luetje
The Molecular Basis for Ligand Specificity in a Mouse Olfactory Receptor: A NETWORK OF FUNCTIONALLY IMPORTANT RESIDUES
J. Biol. Chem., January 12, 2007; 282(2): 1216 - 1224.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. M. D. Santos, L. A. Gardner, S. W. White, and S. W. Bahouth
Characterization of the Residues in Helix 8 of the Human beta1-Adrenergic Receptor That Are Involved in Coupling the Receptor to G Proteins
J. Biol. Chem., May 5, 2006; 281(18): 12896 - 12907.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
N. Terada, N. Ohno, H. Ohguro, Z. Li, and S. Ohno
Immunohistochemical Detection of Phosphorylated Rhodopsin in Light-exposed Retina of Living Mouse with In Vivo Cryotechnique
J. Histochem. Cytochem., April 1, 2006; 54(4): 479 - 486.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Wang, X.-H. Wen, Z. Ablonczy, R. K. Crouch, C. L. Makino, and J. Lem
Enhanced Shutoff of Phototransduction in Transgenic Mice Expressing Palmitoylation-deficient Rhodopsin
J. Biol. Chem., July 1, 2005; 280(26): 24293 - 24300.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
D. M. Perez and S. S. Karnik
Multiple Signaling States of G-Protein-Coupled Receptors
Pharmacol. Rev., June 1, 2005; 57(2): 147 - 161.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Tsukamoto, A. Terakita, and Y. Shichida
A rhodopsin exhibiting binding ability to agonist all-trans-retinal
PNAS, May 3, 2005; 102(18): 6303 - 6308.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
A. Chinen, Y. Matsumoto, and S. Kawamura
Reconstitution of Ancestral Green Visual Pigments of Zebrafish and Molecular Mechanism of Their Spectral Differentiation
Mol. Biol. Evol., April 1, 2005; 22(4): 1001 - 1010.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Chinen, Y. Matsumoto, and S. Kawamura
Spectral Differentiation of Blue Opsins between Phylogenetically Close but Ecologically Distant Goldfish and Zebrafish
J. Biol. Chem., March 11, 2005; 280(10): 9460 - 9466.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
I. D. Alves, G. F. J. Salgado, Z. Salamon, M. F. Brown, G. Tollin, and V. J. Hruby
Phosphatidylethanolamine Enhances Rhodopsin Photoactivation and Transducin Binding in a Solid Supported Lipid Bilayer as Determined Using Plasmon-Waveguide Resonance Spectroscopy
Biophys. J., January 1, 2005; 88(1): 198 - 210.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Zhu, G.-F. Jang, B. Jastrzebska, S. Filipek, S. E. Pearce-Kelling, G. D. Aguirre, R. E. Stenkamp, G. M. Acland, and K. Palczewski
A Naturally Occurring Mutation of the Opsin Gene (T4R) in Dogs Affects Glycosylation and Stability of the G Protein-coupled Receptor
J. Biol. Chem., December 17, 2004; 279(51): 53828 - 53839.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Ritter, K. Zimmermann, M. Heck, K. P. Hofmann, and F. J. Bartl
Transition of Rhodopsin into the Active Metarhodopsin II State Opens a New Light-induced Pathway Linked to Schiff Base Isomerization
J. Biol. Chem., November 12, 2004; 279(46): 48102 - 48111.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Zimmermann, E. Ritter, F. J. Bartl, K. P. Hofmann, and M. Heck
Interaction with Transducin Depletes Metarhodopsin III: A REGULATED RETINAL STORAGE IN VISUAL SIGNAL TRANSDUCTION?
J. Biol. Chem., November 12, 2004; 279(46): 48112 - 48119.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. A. Gascon and V. S. Batista
QM/MM Study of Energy Storage and Molecular Rearrangements Due to the Primary Event in Vision
Biophys. J., November 1, 2004; 87(5): 2931 - 2941.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Chan, A. Bradley, T. G. Wensel, and J. H. Wilson
Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy
PNAS, June 15, 2004; 101(24): 9109 - 9114.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Herrmann, M. Heck, P. Henklein, P. Henklein, C. Kleuss, K. P. Hofmann, and O. P. Ernst
Sequence of Interactions in Receptor-G Protein Coupling
J. Biol. Chem., June 4, 2004; 279(23): 24283 - 24290.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Shi and J. A. Javitch
The second extracellular loop of the dopamine D2 receptor lines the binding-site crevice
PNAS, January 13, 2004; 101(2): 440 - 445.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
O. Man, Y. Gilad, and D. Lancet
Prediction of the odorant binding site of olfactory receptor proteins by human-mouse comparisons
Protein Sci., January 1, 2004; 13(1): 240 - 254.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Krebs, P. C. Edwards, C. Villa, J. Li, and G. F. X. Schertler
The Three-dimensional Structure of Bovine Rhodopsin Determined by Electron Cryomicroscopy
J. Biol. Chem., December 12, 2003; 278(50): 50217 - 50225.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. E. Knox, E. Salcedo, K. Mathiesz, J. Schaefer, W.-H. Chou, L. V. Chadwell, W. C. Smith, S. G. Britt, and R. B. Barlow
Heterologous Expression of Limulus Rhodopsin
J. Biol. Chem., October 17, 2003; 278(42): 40493 - 40502.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Natochin, K. G. Gasimov, M. Moussaif, and N. O. Artemyev
Rhodopsin Determinants for Transducin Activation: A GAIN-OF-FUNCTION APPROACH
J. Biol. Chem., September 26, 2003; 278(39): 37574 - 37581.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. A. Boucard, M. Roy, M.-E. Beaulieu, P. Lavigne, E. Escher, G. Guillemette, and R. Leduc
Constitutive Activation of the Angiotensin II Type 1 Receptor Alters the Spatial Proximity of Transmembrane 7 to the Ligand-binding Pocket
J. Biol. Chem., September 19, 2003; 278(38): 36628 - 36636.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. C. Y. Yan, M. A. Kazmi, Z. Ganim, J.-M. Hou, D. Pan, B. S. W. Chang, T. P. Sakmar, and R. A. Mathies
Retinal counterion switch in the photoactivation of the G protein-coupled receptor rhodopsin
PNAS, August 5, 2003; 100(16): 9262 - 9267.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
B. S. W. Chang
Ancestral Gene Reconstruction and Synthesis of Ancient Rhodopsins in the Laboratory
Integr. Comp. Biol., August 1, 2003; 43(4): 500 - 507.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Fritze, S. Filipek, V. Kuksa, K. Palczewski, K. P. Hofmann, and O. P. Ernst
Role of the conserved NPxxY(x)5,6F motif in the rhodopsin ground state and during activation
PNAS, March 4, 2003; 100(5): 2290 - 2295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. J. del Valle, E. Ramon, X. Canavate, P. Dias, and P. Garriga
Zinc-induced Decrease of the Thermal Stability and Regeneration of Rhodopsin
J. Biol. Chem., February 7, 2003; 278(7): 4719 - 4724.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-i. Miura, J. Zhang, J. Boros, and S. S. Karnik
TM2-TM7 Interaction in Coupling Movement of Transmembrane Helices to Activation of the Angiotensin II Type-1 Receptor
J. Biol. Chem., January 31, 2003; 278(6): 3720 - 3725.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. L. Meacock, F. J.L. Lecomte, S. G. Crawshaw, and S. High
Different Transmembrane Domains Associate with Distinct Endoplasmic Reticulum Components during Membrane Integration of a Polytopic Protein
Mol. Biol. Cell, December 1, 2002; 13(12): 4114 - 4129.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Vogel, G.-B. Fan, S. Ludeke, F. Siebert, and M. Sheves
A Nonbleachable Rhodopsin Analogue with a Slow Photocycle
J. Biol. Chem., October 18, 2002; 277(43): 40222 - 40228.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
B. S. W. Chang, K. Jonsson, M. A. Kazmi, M. J. Donoghue, and T. P. Sakmar
Recreating a Functional Ancestral Archosaur Visual Pigment
Mol. Biol. Evol., September 1, 2002; 19(9): 1483 - 1489.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. W. Kijas, A. V. Cideciyan, T. S. Aleman, M. J. Pianta, S. E. Pearce-Kelling, B. J. Miller, S. G. Jacobson, G. D. Aguirre, and G. M. Acland
Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa
PNAS, April 30, 2002; 99(9): 6328 - 6333.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. W. Kijas, A. V. Cideciyan, T. S. Aleman, M. J. Pianta, S. E. Pearce-Kelling, B. J. Miller, S. G. Jacobson, G. D. Aguirre, and G. M. Acland
Naturally occurring rhodopsin mutation in the dog causes retinal dysfunction and degeneration mimicking human dominant retinitis pigmentosa
PNAS, April 30, 2002; 99(9): 6328 - 6333.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online